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Nkx2.5 Antibody

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

P42582

Host

Rabbit

Reactivity

Mouse

Immunogen

This mouse Nkx2.5 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 192-235 amino acids from the Central region of the mouse protein.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Nkx2.5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Nkx2.5 antibody is available for research use only.

Storage Conditions

Aliquot the Nkx2.5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Nkx2.5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Rat

Image Legend

Western blot analysis of lysate from mouse stomach tissue lysate using Nkx2.5 antibody at 1:1000.

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