Shh Antibody / Sonic Hedgehog

Sonic hedgehog binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow Cytometry: 1:25, Western blot: 1:1000 (1)

UniProt

Q62226

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 395-450 were used as immunogen for this Shh antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Shh antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Shh antibody is available for research use only.

Storage Conditions

Aliquot the Shh antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Shh antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The 45 kDa precursor protein autocleaves into 27 kDa amino and 19 kDa carboxy fragments.