EpCAM Antibody

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity) .

Product Specifications

CAS Number

9007-83-4

Specifications

Flow Cytometry: 1:25, Western blot: 1:1000

UniProt

Q99JW5

Host

Rabbit

Reactivity

Mouse, Human

Immunogen

This mouse Epcam antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 302-335 amino acids from the C-terminal region of mouse Epcam.

Clonality

Polyclonal

Isotype

Applications

FACS, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the EpCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This EpCAM antibody is available for research use only.

Storage Conditions

Aliquot the EpCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the EpCAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Rat

Image Legend

Flow cytometric analysis of HepG2 cells using Epcam antibody (green) compared to an isotype control of rabbit IgG (blue) ; Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG was used as the secondary Ab.