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ZO1 Antibody

The N-terminal of ZO1 may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

Q07157

Host

Rabbit

Reactivity

Human

Immunogen

This ZO1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 1328-1362 amino acids from the C-terminal region of human TJP1/ZO1.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the ZO1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ZO1 antibody is available for research use only.

Storage Conditions

Aliquot the ZO1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the ZO1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of lysate from A431 cell line using ZO1 antibody diluted at 1:1000.

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