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SMAD2 Antibody

SMAD2 is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000

UniProt

Q62432

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

This SMAD2 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 198-232 amino acids of mouse Smad2.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the SMAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SMAD2 antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the SMAD2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the SMAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Pig, Chicken

Image Legend

Western blot analysis of lysate from (1) HeLa, (2) mouse NIH3T3, (3) mouse C2C12, and (4) rat PC-12 cell line using SMAD2 antibody at 1:1000. Predicted molecular weight: 52~60 kDa.

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