CD34 Antibody

CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

UniProt

P28906

Host

Rabbit

Reactivity

Human

Immunogen

This CD34 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 320-354 amino acids from the C-terminal region of human CD34.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the CD34 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD34 antibody is available for research use only.

Storage Conditions

Aliquot the CD34 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the CD34 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse

Image Legend

Western blot analysis of lysate from KG-1 cell line using CD34 antibody diluted at 1:1000. Expected size is 40~110 kDa depending on glycosylation level.