SIRT1 Antibody

SIRT1 is a NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

IHC (Paraffin) : 1:25, Immunofluorescence: 1:25, Western blot: 1:1000

UniProt

Q923E4

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 566-601 of the mouse protein was used as the immunogen for the SIRT1 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, IF, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the SIRT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SIRT1 antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the SIRT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the SIRT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of lysate from mouse F9 cell line using SIRT1 antibody at 1:1000. Predicted molecular weight ~80kDa but is routinely observed at 110~120kDa due to post-translational modification.