Zebrafish Gapdh Antibody
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins (By similarity) .
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
Q5XJ10
Host
Rabbit
Reactivity
Human, Zebrafish
Immunogen
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the Zebrafish Gapdh antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Zebrafish Gapdh antibody is available for research use only.
Storage Conditions
Aliquot the Zebrafish Gapdh antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Zebrafish Gapdh antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from HUVEC cell line using Gapdh antibody. Ab was diluted at 1:1000. Predicted molecular weight ~36kDa.
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