SMAD1 Antibody
SMADs are intracellular proteins that transduce extracellular signals from transforming growth factor beta ligands to the nucleus where they activate downstream gene transcription. The SMADs, which form a trimer of two receptor-regulated SMADs and one co-SMAD, act as transcription factors that regulate the expression of certain genes. There are three classes of SMADs, SMAD1 belongs to the receptor-regulated class (R-SMAD) which includes SMAD2, SMAD3, SMAD5 and SMAD8/9. [Wiki]
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:25, IHC (Paraffin) : 1:25, Western blot: 1:1000
UniProt
Q15797
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, IF, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the SMAD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SMAD1 antibody is available for research use only.
Storage Conditions
Aliquot the SMAD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the SMAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine
Image Legend
IHC analysis of FFPE human heart section using SMAD1 antibody; Ab was diluted at 1:25.
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