DHFR Antibody
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
Product Specifications
CAS Number
9007-83-4
Specifications
IHC (Paraffin) : 1:25, Western blot: 1:1000
UniProt
P00374
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A portion of amino acids 1-50 from human Dihydrofolate reductase was used as the immunogen for this DHFR antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the DHFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This DHFR antibody is available for research use only.
Storage Conditions
Aliquot the DHFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the DHFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, Pig
Image Legend
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