DHFR Antibody

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.

Product Specifications

CAS Number

9007-83-4

Specifications

IHC (Paraffin) : 1:25, Western blot: 1:1000

UniProt

P00374

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 1-50 from human Dihydrofolate reductase was used as the immunogen for this DHFR antibody.

Clonality

Polyclonal

Isotype

Applications

IHC, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the DHFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This DHFR antibody is available for research use only.

Storage Conditions

Aliquot the DHFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the DHFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Pig

Image Legend

Western blot analysis of lysate from 1) 293T, 2) HeLa, 3) Jurkat, 4) Y79 cell line , 5) mouse spleen and 6) rat kidney tissue lysate using DHFR antibody at 1:1000. Predicted molecular weight ~22 kDa.