SP1 Antibody / Specificity Protein 1
Specificity Protein 1/SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation) . Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Flow Cytometry: 1:25, Immunofluorescence: 1:25, Western blot: 1:1000
UniProt
P08047
Host
Mouse
Reactivity
Human
Immunogen
This SP1 antibody was produced from a mouse immunized with recombinant Specificity Protein 1.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
1326CT463.109.176
Applications
WB, FACS, IF, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the SP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SP1 antibody is available for research use only.
Storage Conditions
Aliquot the SP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the SP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from human (1) HeLa and (2) Ramos cell line using SP1 antibody at 1:1000. Reported molecular weight: 81-95 kDa.
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