PCNA Antibody (Proliferating Cell Nuclear Antigen)
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Product Specifications
CAS Number
9007-83-4
Specifications
IHC (Paraffin) : 1:25, Western blot: 1:2000
UniProt
P12004
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A portion of amino acids 236-261 from human Proliferating Cell Nuclear Antigen was used as the immunogen for this PCNA antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
1157CT1.1.3
Applications
IHC, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the PCNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PCNA antibody is available for research use only.
Storage Conditions
Aliquot the PCNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the PCNA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, Hamster
Image Legend
Immunohistochemical analysis of paraffin-embedded human lymph using PCNA antibody at 1:25 dilution.
Documents
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