SUMO-2 Antibody

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Western blot: 1:1000

UniProt

P61956

Host

Mouse

Reactivity

Human, Rat

Immunogen

Purified His-tagged protein was used to produced this monoclonal SUMO-2 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

973CT8.1.1

Applications

IF, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the SUMO-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SUMO-2 antibody is available for research use only.

Storage Conditions

Aliquot the SUMO-2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the SUMO-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Chicken, Hamster, Mouse, Pig

Image Legend

Fluorescent image of HeLa cells stained with SUMO-2 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green) . Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red) .