UCHL1 Antibody
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Product Specifications
CAS Number
9007-83-4
Specifications
IHC (Paraffin) : 1:25, Western blot: 1:1000
UniProt
P09936
Host
Mouse
Reactivity
Human, Rat
Immunogen
This UCHL1 antibody was produced from mice immunized with a KLH conjugated synthetic peptide selected from the 185-214 region of human UCHL1.
Clonality
Monoclonal
Isotype
IgG1
Clone
696CT2.1.4
Applications
IHC, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the UCHL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This UCHL1 antibody is available for research use only.
Storage Conditions
Aliquot the UCHL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the UCHL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Bovine, Mouse, Pig
Image Legend
UCHL1 antibody western blot analysis in (1) 293T, (2) NCI-H1299 and (3) rat PC-12 lysate. Predicted molecular weight ~25 kDa.
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