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UCHL1 Antibody

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Product Specifications

CAS Number

9007-83-4

Specifications

IHC (Paraffin) : 1:25, Western blot: 1:1000

UniProt

P09936

Host

Mouse

Reactivity

Human, Rat

Immunogen

This UCHL1 antibody was produced from mice immunized with a KLH conjugated synthetic peptide selected from the 185-214 region of human UCHL1.

Clonality

Monoclonal

Isotype

IgG1

Clone

696CT2.1.4

Applications

IHC, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the UCHL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This UCHL1 antibody is available for research use only.

Storage Conditions

Aliquot the UCHL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the UCHL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Mouse, Pig

Image Legend

UCHL1 antibody western blot analysis in (1) 293T, (2) NCI-H1299 and (3) rat PC-12 lysate. Predicted molecular weight ~25 kDa.

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