TBP Antibody / TATA-box-binding protein

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.

Product Specifications

CAS Number

9007-83-4

Specifications

IHC (Paraffin) : 1:25, Western blot: 1:1000-2:000

UniProt

P20226

Host

Mouse

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Purified His-tagged protein was used to produced this monoclonal TBP antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

830CT4.3.3

Applications

IHC-P, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TBP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TBP antibody is available for research use only.

Storage Conditions

Aliquot the TBP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the TBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

Western blot testing of 1) human 293, 2) (h) HeLa, 3) (h) MDA-MB-231, 4) rat H-4-II-E, 5) (h) HCT-116 and 6) mouse C2C12 cell lysate with TBP antibody at 1:2000. Expected molecular weight: 35-43 kDa.