Anti-p21 Antibody
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex (By similarity) .
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
P39689
Host
Rabbit
Reactivity
Mouse
Immunogen
A portion of amino acids 126-154 from the mouse protein was used as the immunogen for this anti-p21 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the anti-p21 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This anti-p21 antibody is available for research use only.
Storage Conditions
Aliquot the anti-p21 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the anti-p21 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Anti-p21 antibody western blot analysis in mouse NIH3T3 lysate
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