His Tag Antibody
Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. NSJBio's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
Host
Rabbit
Immunogen
Poly-His peptide was used as the immungen for the His Tag antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the His Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This His Tag antibody is available for research use only.
Storage Conditions
Aliquot the His Tag antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of 1) 293T/17 cell lysate, 2) 12-tag transfected 293T/17 cell lysate and 3) 12-tag recombinant protein with His Tag antibody.
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