Anti-GST Antibody

Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the NSJBio anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

Host

Rabbit

Immunogen

Recombinant full length GST was used as the immunogen for this anti-GST antibody.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the anti-GST antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This anti-GST antibody is available for research use only.

Storage Conditions

Aliquot the anti-GST antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the anti-GST antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Anti-GST antibody used in western blot to detect a GST-tagged recombinant protein purified from bacterial lysate (Lanes 1-3: 10, 40, and 160 ng protein) .