Anti-His Tag Antibody
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
Host
Rabbit
Immunogen
HHHHHH peptide was used as the immunogen for the anti-His Tag antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the anti-His Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This anti-His Tag antibody is available for research use only.
Storage Conditions
Aliquot the anti-His Tag antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the anti-His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of anti-His Tag antibody and a tagged recombinant protein.
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