V5 Tag Antibody

Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5) . NSJBio's anti-V5 tag polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000

Host

Rabbit

Immunogen

The V5 sequence GKPIPNPLLGLDST was used as the immunogen for the V5 tag antibody.

Clonality

Polyclonal

Isotype

Applications

WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the V5 Tag antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This V5 Tag antibody is available for research use only.

Storage Conditions

Aliquot the V5 Tag antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the V5 Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Lanes: 1: non-transfected cells; 2: V5-tagged empty plasmid; and V5-tagged proteins: 3-A, 4-B, 5-C, 6-D; Data courtesy of Dr. Gustavo Gutierrez, Burnham Institute for Medical Research.