FLAG Antibody
Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
Host
Rabbit
Immunogen
The sequence cDYKDDDDK was used as the immunogen for this FLAG antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the FLAG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This FLAG antibody is available for research use only.
Storage Conditions
Aliquot the FLAG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the FLAG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from a 41 kDa 12 tagged protein using FLAG antibody at 1:1000.
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