HA Antibody
Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. NSJBio's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Immunofluorescence: 1:10-1:50, ChIP: 1:100, Immunoprecipitation: suitable
Host
Rabbit
Immunogen
The sequence CYPYDVPDYAYPYDVPDYA was used as the immunogen for this HA antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IF, IP, ChIP, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the HA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HA antibody is available for research use only.
Storage Conditions
Aliquot the HA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the HA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from 12 tagged recombinant protein cell lines using HA antibody diluted at 1:1000.
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