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HA Antibody

Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. NSJBio's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50, ChIP: 1:100, Immunoprecipitation: suitable

Host

Rabbit

Immunogen

The sequence CYPYDVPDYAYPYDVPDYA was used as the immunogen for this HA antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IF, IP, ChIP, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the HA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HA antibody is available for research use only.

Storage Conditions

Aliquot the HA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the HA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of lysate from 12 tagged recombinant protein cell lines using HA antibody diluted at 1:1000.

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