GST Antibody
Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the NSJBio anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:100-1:500
Host
Mouse
Immunogen
Purified recombinant fusion protein was used to produced this monoclonal GST antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
21CT54.13.1
Applications
WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the GST antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This GST antibody is available for research use only.
Storage Conditions
Aliquot the GST antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the GST antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of GST antibody and recombinant protein.
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Frequently Asked Questions
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