Anti-His Antibody
Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18, provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. NSJBio's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000-1:4000
Host
Mouse
Immunogen
Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal anti-His antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
6AT18
Applications
WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Store at 4oC for up to one month. For long-term, aliquot the anti-His antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This anti-His antibody is available for research use only.
Storage Conditions
Store at 4°C for up to one month. For long-term, aliquot the anti-His antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Image Legend
Western blot analysis of lysate from 12 tag recombinant protein using anti-His antibody at 1:1000.
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