Anti-His Antibody

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18, provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. NSJBio's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-1:4000

Host

Mouse

Immunogen

Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal anti-His antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

6AT18

Applications

WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long-term, aliquot the anti-His antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This anti-His antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long-term, aliquot the anti-His antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the anti-His antibody may be required due to differences in protocols and secondary/substrate sensitivity.