Anti-HA Antibody
Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. NSJ's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Immunoprecipitation: published application
Host
Mouse
Immunogen
KLH conjugated synthetic peptide cYPYDVPDYA was used as the immunogen for the anti-HA antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
12CA5
Applications
WB, IP, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the anti-HA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This anti-HA antibody is available for research use only.
Storage Conditions
Aliquot the anti-HA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the anti-HA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Anti-HA tag antibody testing a tagged recombinant protein.
Frequently Asked Questions
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Caprisil C1 HPLC Column, 5 µm, 100 Å, CW553-C1 x 300 mm