C-Myc Antibody
MYC is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. It activates the transcription of growth-related genes [UniProt]. It is also commonly used as an epitope tag to purifiy and identify tagged recombinant proteins.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000-4000, FACS: assay dependent, IHC (Paraffin) : assay dependent
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 408-439 of the human protein were used as the immunogen for the c-Myc antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
9E10.
Applications
WB, FACS, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the c-Myc antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This c-Myc antibody is available for research use only.
Storage Conditions
Aliquot the c-Myc antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the c-Mycantibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from 12-Tag protein using c-Myc antibody; Ab was diluted at 1:4000.
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