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C-Myc Antibody

MYC is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. It activates the transcription of growth-related genes [UniProt]. It is also commonly used as an epitope tag to purifiy and identify tagged recombinant proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000-4000, FACS: assay dependent, IHC (Paraffin) : assay dependent

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 408-439 of the human protein were used as the immunogen for the c-Myc antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

9E10.

Applications

WB, FACS, IHC, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the c-Myc antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This c-Myc antibody is available for research use only.

Storage Conditions

Aliquot the c-Myc antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the c-Mycantibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of lysate from 12-Tag protein using c-Myc antibody; Ab was diluted at 1:4000.

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