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Src Antibody

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-Src kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:2000, IHC (Paraffin) : 1:50, Flow Cytometry: 1:50, Immunofluorescence: 1:50

UniProt

P12931

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 24-52 from the human protein was used as the immunogen for this Src antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, FACS, IF, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Src antibody is available for research use only.

Storage Conditions

Aliquot the Src antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse

Image Legend

Src antibody western blot analysis in (1) MCF-7 and (2) A431 lysate

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