Vimentin Antibody
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL) -derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:2000, IHC (Paraffin) : 1:50, Flow Cytometry: 1:50, Immunofluorescence: 1:50
UniProt
P08670
Host
Rabbit
Reactivity
Human
Immunogen
This Vimentin antibody was produced from rabbits immunized with human Vimentin recombinant protein.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, WB, IHC, FACS, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Vimentin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Vimentin antibody is available for research use only.
Storage Conditions
Aliquot the Vimentin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Vimentin antibody western blot analysis in 1) HeLa, 2) U251, 3) A549, and 4) MDA-MB231 lysate
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