SMAD3 Antibody
SMAD3 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50
UniProt
P84022
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 186-215 from the human protein was used as the immunogen for this SMAD3 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, IF, FACS, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the SMAD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SMAD3 antibody is available for research use only.
Storage Conditions
Aliquot the SMAD3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Rat, Pig, Chicken
Image Legend
SMAD3 antibody western blot analysis in HepG2 and mouse C2C12 lysate. Observed molecular weight: 48~55 kDa.
Frequently Asked Questions
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