NEDD4 Antibody

Nedd4 is one of a group of mouse genes that show developmentally regulated expression in mouse embryonic brain. Nedd4 is expressed in various other embryonic tissues and persists in most adult tissues.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

UniProt

P46934

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 1288-1319 from the human protein was used as the immunogen for this NEDD4 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the NEDD4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NEDD4 antibody is available for research use only.

Storage Conditions

Aliquot the NEDD4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the NEDD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Rat

Image Legend

Western blot analysis of NEDD4 antibody and mouse NIH3T3 lysate. Predicted molecular weight ~149 kDa; commonly observed at 110-149 kDa with a possible ~95 kDa cleavage band.