ATG13 Antibody

ATG13 is an autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

UniProt

O75143

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for this ATG13 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ATG13 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ATG13 antibody is available for research use only.

Storage Conditions

Aliquot the ATG13 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the ATG13 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine

Location

Cytoplasmic

Image Legend

ATG13 antibody western blot analysis in 1) MDA-MB435,2) CEM, 3) T47D cell line and 4) mouse cerebellum tissue lysate. Predicted molecular weight ~57kDa.