Phospholamban Antibody

PLB is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR) . PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

UniProt

P26678

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for the Phospholamban antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Phospholamban antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Phospholamban antibody is available for research use only.

Storage Conditions

Aliquot the Phospholamban antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the Phospholamban antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Phospholamban antibody flow cytometric analysis of Jurkat cells (green) compared to a negative control (blue) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.