AIM2 Antibody
Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity) . Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50
UniProt
O14862
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 1-50 from the human protein was used as the immunogen for this AIM2 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, FACS, IF, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the AIM2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This AIM2 antibody is available for research use only.
Storage Conditions
Aliquot the AIM2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the AIM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, membrane, nuclear
Image Legend
Western blot analysis of AIM2 antibody and 1) MCF-7, 2) HL-60, 3) K562 and 4) HeLa lysate. Predicted molecular weight 40-45 kDa.
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