RNF8 Antibody

RNF8 contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50

UniProt

O76064

Host

Rabbit

Reactivity

Human

Immunogen

This RNF8 antibody was produced from rabbits immunized with recombinant protein of human RNF8.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the RNF8 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This RNF8 antibody is available for research use only.

Storage Conditions

Aliquot the RNF8 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the RNF8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of RNF8 antibody and Jurkat lysate. Predicted molecular weight ~56 kDa but may be observed at larger sizes due to ubiquitination.