TLR4 Antibody

TLR4 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50

UniProt

O00206

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 669-698 from the human protein was used as the immunogen for this TLR4 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the TLR4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TLR4 antibody is available for research use only.

Storage Conditions

Aliquot the TLR4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the TLR4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Pig, Hamster

Image Legend

TLR4 antibody flow cytometric analysis of U251 cells (right histogram) compared to a negative control (left histogram) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.