ChAT Antibody
ChAT (Choline acetyltransferase) is an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Immunofluorescence: 1:10-1:50
UniProt
P28329
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 98-128 from the human protein was used as the immunogen for this ChAT antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the CHAT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This ChAT antibody is available for research use only.
Storage Conditions
Aliquot the ChAT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the ChAT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of ChAT antibody and NCI-H460 lysate. Predicted molecular weight ~83/74 kDa (isoforms M/S) .
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