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ChAT Antibody

ChAT (Choline acetyltransferase) is an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50

UniProt

P28329

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 98-128 from the human protein was used as the immunogen for this ChAT antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IF, WB, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS pH 7.4 with 0.09% sodium azide

Reconstitution

Aliquot the CHAT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ChAT antibody is available for research use only.

Storage Conditions

Aliquot the ChAT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the ChAT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of ChAT antibody and NCI-H460 lysate. Predicted molecular weight ~83/74 kDa (isoforms M/S) .

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