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LDHA Antibody

LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

UniProt

P00338

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 154-182 from the human protein was used as the immunogen for this LDHA antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, IHC, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the LDHA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This LDHA antibody is available for research use only.

Storage Conditions

Aliquot the LDHA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the LDHA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Pig, Rat

Location

Cytoplasmic, nuclear

Image Legend

LDHA antibody western blot analysis in 1) Ramos and 2) A375 lysate. Predicted molecular weight ~36kDa.

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