CEBPA Antibody

CEBPA is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. This protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Flow Cytometry: 1:10-1:50

UniProt

P49715

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 304-330 from the human protein was used as the immunogen for this CEBPA antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, WB, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CEBPA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CEBPA antibody is available for research use only.

Storage Conditions

Aliquot the CEBPA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the CEBPA antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Bovine, Rat

Image Legend

CEBPA antibody flow cytometric analysis of HeLa cells (green) compared to a negative control (blue) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.