HNF4A Antibody

HNF4A is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, Immunofluorescence: 1:10-1:50

UniProt

P41235

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 107-139 from the human protein was used as the immunogen for this HNF4A antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HNF4A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HNF4A antibody is available for research use only.

Storage Conditions

Aliquot the HNF4A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the HNF4A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat, Xenopus

Location

Predominantly nuclear, present in cytoplasm in cells with high HNF4A expression

Image Legend

Confocal immunofluorescent analysis of HNF4A antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .