ANGPTL4 Antibody

ANGPTL4 is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This protein is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. The encoded protein is a serum hormone directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity and also acts as an apoptosis survival factor for vascular endothelial cells. The encoded protein may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

UniProt

Q9BY76

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 138-167 from the human protein was used as the immunogen for this ANGPTL4 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the ANGPTL4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ANGPTL4 antibody is available for research use only.

Storage Conditions

Aliquot the ANGPTL4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the ANGPTL4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot analysis of ANGPTL4 antibody and mouse liver tissue lysate. Predicted molecular weight: ~45 kDa and ~41 kDa, but may be observed at higher molecular weights due to glycosylation.