HMGA1 Antibody
HMGA1 encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1:10-1:50, Western blot: 1:1000, Flow Cytometry: 1:10-1:50
UniProt
P17096
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 64-93 from the human protein was used as the immunogen for this HMGA1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, IF, WB, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the HMGA1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HMGA1 antibody is available for research use only.
Storage Conditions
Aliquot the HMGA1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the HMGA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat, Hamster
Image Legend
Fluorescent image of A549 cell stained with HMGA1 antibody at 1:25. HMGA1 immunoreactivity is localized to the nucleus.
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