PKM2 Antibody

PKM2 encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

UniProt

P14618

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 116-145 from the human protein was used as the immunogen for this PKM2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, FACS, ELISA

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PKM2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PKM2 antibody is available for research use only.

Storage Conditions

Aliquot the PKM2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Prediction Reactivity

Mouse, Rat, Rabbit

Image Legend

PKM2 antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control (left histogram) . FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.