NKX3.1 Antibody

NKX3.1 is a transcription factor which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

UniProt

Q99801

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 118-145 from the human protein was used as the immunogen for this NKX3.1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC, FACS, ELISA

Purity

Antigen affinity

Format

Antigen affinity purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Store at 4oC for up to one month. For long term, aliquot the NKX3.1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NKX3.1 antibody is available for research use only.

Storage Conditions

Store at 4°C for up to one month. For long term, aliquot the NKX3.1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the NKX3.1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.