HuR Antibody
ELAVL1/HuR is involved in 3'-UTR ARE-mediated MYC stabilization. It binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50
UniProt
Q15717
Host
Rabbit
Reactivity
Human
Immunogen
This HuR antibody was produced from rabbits immunized with human ELAVL1/HuR recombinant protein.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, WB, IHC, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the HuR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HuR antibody is available for research use only.
Storage Conditions
Aliquot the HuR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the HuR antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of HuR antibody and HeLa, Jurkat, CEM, Ramos lysate.
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