HIST3H3 Antibody (Histone H3.1t)
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
Q16695
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 1-30 from human HISTH3H was used as the immunogen for this HIST3H3 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS pH 7.4 with 0.09% sodium azide
Reconstitution
Aliquot the Histone HIST3H3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This HIST3H3 antibody is available for research use only.
Storage Conditions
Aliquot the Histone HIST3H3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the Histone HIST3H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Mouse, Rat, Bovine, Pig, Chicken, Zebrafish, Xenopus, Drosophila, C. elegans
Image Legend
Western blot analysis of HIST3H3 antibody and (1) CEM, (2) K562, and (3) HL-60 lysate
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